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KMID : 1161420120150070611
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Journal of Medicinal Food
2012 Volume.15 No. 7 p.611 ~ p.620
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Rosmarinic Acid Content in Antidiabetic Aqueous Extract of Ocimum canum Sims Grown in Ghana
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Berhow Mark A.
Affum Andrews Obeng
Gyan Ben A.
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Abstract
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Rosmarinic acid (RA) is an important antioxidant polyphenol that is found in a variety of spices and herbs, including Ocimum canum Sims (locally called eme or akokobesa in Ghana). Aqueous extracts from the leaves of O. canum are used as an antidiabetic herbal medicine in Ghana. Analytical thin-layer chromatography was used to examine the composition of the polyphenols in leaf extracts. The polyphenol content in the aqueous and methanol extracts from the leaf, as determined by the Folin?Ciocalteu method, were 314 and 315?mg gallic acid equivalent/g leaf sample, respectively. The total flavonoid concentration as determined by the aluminum(III) chloride method was 135?mg catechin equivalent/g leaf sample. High-performance liquid chromatography coupled to an electrospray Quadrupole time-of-flight mass spectrometer was also used to determine the polyphenol fingerprint profile in the leaf extracts of O. canum. Although the average RA concentration in the O. canum leaf extracts from Ghana was 1.69?mg/g dry weight (reported values range from 0.01 to 99.62?mg/g dry weight), this polyphenol was still a prominent peak in addition to caffeic acid derivatives.
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KEYWORD
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anti-diabetic drug, aluminum(III) chloride method, flavonoids, Folin?Ciocalteu method, HPLC-ESI-MS, leaves, Ocimum canum Sims, polyphenol profile, rosmarinic acid, thin layer chromatography
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